CHROMATOGRAPHIC FINGERPRINTING AND CLUSTERING OF PLANTAGO MAJOR L. FROM DIFFERENT AREAS IN INDONESIA

., Kartini and ., Azminah (2012) CHROMATOGRAPHIC FINGERPRINTING AND CLUSTERING OF PLANTAGO MAJOR L. FROM DIFFERENT AREAS IN INDONESIA. Asian Journal of Pharmaceutical and Clinical Research, 5 (4). pp. 191-195. ISSN 0974-2441

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Abstract

Plantago major L. is a ubiquitous herbaceous plant with many medicinal activities which has been extensively used in Indonesian traditional medicine. This plant grows at wide range of environment, has several subspecies and varieties, but could not be distinguished morphologically. It is important to develop an effective method for identification and quality assurance of P. major, thus the final product has reproducible quality. In this research, a chromatographic fingerprint method was developed for exploring and establishing the variation of chemical substances among different samples of P. major collected from 15 areas in Indonesia. The LC (liquid chromatography) data showed considerable variation of chemicals among P. major samples. Three chemo-types were visually developed from the LC profiles. The hierarchical clustering analysis also concluded that the samples were divided into three major clusters. Furthermore, the bio-active marker aucubin in this herb was quantitatively determined by a validated LC analysis. Chemo-type II and III were identified as “compound 1”-rich and aucubin-relatively rich chemo-types. These conclusions provide an important basis to establish good agriculture practice and select geo-authentic crude drug for P. major in Indonesia. The validated method was concluded to be suitable for fingerprint analysis for the quality control of P. major

Item Type: Article
Uncontrolled Keywords: Plantago major, Chromatographic fingerprinting, Clustering analysis, Aucubin, Column liquid chromatography
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Eko Setiawan 194014
Date Deposited: 17 Oct 2012 06:32
Last Modified: 09 Jun 2017 06:47
URI: http://repository.ubaya.ac.id/id/eprint/1192

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