Increasing of Carotene Production from Local Isolate when using Additional Supernatant from Bacterial Culture in Their Growth Medium

Putra, Sulistyo Emantoko Dwi and Tjandra, Paulina and Wonohadi, Elisawati (2008) Increasing of Carotene Production from Local Isolate when using Additional Supernatant from Bacterial Culture in Their Growth Medium. Journal of Applied and Industrial Biotechnology in Tropical Region, 1. pp. 1-3. ISSN 1979-9748

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Abstract

Screening of quorum sensing(QS) quencher takes many attention because of resistances problems in the using of antibiotics to kill pathogenic bacteria. There are several reports that QS mechanism controls more than one metabolism that not very closes relatively each others. Because of these reasons research in finding of quencher molecule should take into account about side effect of the molecule. Chromobacterium violacein Tn5 and lasR-lasl-GFP plasmid recombinant that uses in the QS research seems to be difficult to delect side effect on using. QS quencher molecul, because of engineering on their DNA make the reporter gene express higher than the normal gene, this make difficulties to observe other gene which is effect by quencher molecule. In this research we used wild type local isolate of bacteria that produce carotene. We also reported that carotene production in our isolate was driven by quorum sensing. Adding of supernatant from bacterial culture in bacterial growth medium until 25; 50 and 75% gave in increasing of carotene production until 64; 50 and 50%. Extracting supernatant from bacterial culture using ethyl acetate and adding to the growth medium until 40; 50 and 60%, gave 31; 40 and 22% increasing of carotene production. In this research we reported that adding of supernatant or ethyl acetate axtract supernatant from bacterial culture also decreasing cell mass production. Based oh this data we concluded that increasing of carotene production causing by increasing of carotene production per cell not by increasing if number of cells.

Item Type: Article
Uncontrolled Keywords: quorum sensing, carotene, local isolate
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Perpustakaan UBAYA
Date Deposited: 06 Feb 2012 07:40
Last Modified: 28 Jul 2021 00:09
URI: http://repository.ubaya.ac.id/id/eprint/125

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