Initiation of Callus Cultures of Cantaloupe Melon (Cucumis melo L.) and Detection of Its Beta-Carotene Content

Tjie , Kok and Daniel, Xavier and Kristianti, The Sandy (2013) Initiation of Callus Cultures of Cantaloupe Melon (Cucumis melo L.) and Detection of Its Beta-Carotene Content. In: PROCEEDING INTERNATIONAL CONFERENCE ON ENVIRONMENT AND HEALTH : “INTEGRATING RESEARCH COMMUNITY OUTREACH AND SERVICE LEARNING”. Soegijapranata Catholic University, Semarang, pp. 56-60. ISBN 978-602-8011-53-2

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Abstract

Cantaloupe Melon (Cucumis melo L.) is a fruit plant riching in antioxidant beta-carotene. Perhaps, production of beta-carotene can also be achieved by first initiating callus culture of the plant in suitable medium, propagating them, and extracting the compound it produce. This research was a preliminary effort aimed to examine it. The results of this research showed that the optimum medium for callus culture initiation from cotyledon of Cantaloupe Melon and its propagation was Murashige Skoog (MS) with the addition of 1 mg/L benzyl adenine (BA) and 1.5 mg/L napthalene acetic Acid (NAA). The beginning of stationary phase on calluses’ growth curve was chosen as a harvest time of calluses, based on the theory that beta-carotene as a secondary metabolite is usually be produced much at that phase. The results demonstrated that stationary phase began at the end of week fourth, so the time was decided as the harvest time of calluses. Beta-carotene was then extracted from calluses by maceration technique. The existence of the compound in extract was tested using Thin Layer Chromatography (TLC) and Fourier Transformed-Infrared Spectroscopy (FTIR). The data showed that the compound existed in the extract. The concentration of the compound in it was needed to determine further.

Item Type: Book Section
Uncontrolled Keywords: Cantaloupe Melon, beta-carotene, callus cultures, Thin Layer Chromatography (TLC), Fourier Transformed-Infrared Spectroscopy (FTIR)
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 27 Aug 2014 08:03
Last Modified: 27 Aug 2014 08:04
URI: http://repository.ubaya.ac.id/id/eprint/20384

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