Cloning, Characterization And Overexpression Of The Salmonella Typhi carB Gene

Wahjudi, Mariana and Wonohadi, Elisawati and Rudiretna, Ari (2003) Cloning, Characterization And Overexpression Of The Salmonella Typhi carB Gene. In: Indonesia Toray Science Foundation Seminar on Science and Technology, 3 february 2003, Jakarta.

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Salmonella typhi carAB operon is thought to be intimately involved in the pathogenesis mechanism of typhoid fever disease in human. The carAB operon consist of the carA gene and carB gene which encode two subunits of carbamoyl-phosphate synthetase, small and large subunits. The nucleotide sequence of S. typhi carA gene had been determined (Rudiretna et al, 1998) and its functional studies is still being done. But, information about the structural of the S. typhi carB gene is very little and there was not any information about functional properties of this gene. In order to obtain information related to the structure of this gene, Wahyudi, et. al (2001) had isolated the complete S. typhi carB gene and cloned this gene using the p-GemT vector into E. coli XL10. In this research attempts have been done to clone the S. typhi carB gene into the expression vector, characterize and overexpress the gene. Unfortunately, until now this work has not been succeded yet.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Cloning, Salmonella
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology > QR355 Virology
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Eko Setiawan 194014
Date Deposited: 25 Sep 2014 02:04
Last Modified: 05 Nov 2020 08:54

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