PA0305 attenuates the virulence factors production of Pseudomonas aeruginosa

Wahjudi, Mariana and Poelarends, Gerrit and Quax, Wim J. (2010) PA0305 attenuates the virulence factors production of Pseudomonas aeruginosa. In: Book of Abstracts International Seminar of Indonesian Society for Microbiology. Perhimpunan Mikrobiolgi Indonesia dan IPB, Bogor, p. 66.

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Pseudomonas aeruginosa is a human opportunistic pathogen. It is known that many virulent factors production of the bacteria were modulated by acyl-homoserine lactones as signal. molecules of quorum sensing system. Since acyl-homoserine lactone may also act directly as virulence factors in mammalian hosts, degradation of acyl-homoserine lactone may be interesting not only to stop the quorum sensing system, but also to reduce directly the virulence effects. Previous study proved that P.aeruginosa PA0305 protein is an acylase. It degraded the acyl-homoserine lactone from middle to long acyl-side chain of acyl-homoserine lactone. including N-3-oxo-dodecanoyl-homoserine lactone. one of the P.aeruginosa signal molecules. The aim of this study is to investigate the quenching effect of pa0305 to the quorum sensing system of P.aeruginosa. The over-expression of pa0.305 gene reduced the accumulation of 3-oxo-C12-HSL signal molecule although the accumulation of the other one. N-butanoyl-homoserine lactone, was not changed. In addition. the elastolytic activity were reduced in P. aeruginosa wild type and complemented strains however the pyocyanin production was decreased during the logarithmic phase, not changed after reached the stationary phase. This study showed that the implementation of pa0.305 attenuated the production of some P.aeruginosa virulence factors which were quorum sensing-dependent genes.

Item Type: Book Section
Uncontrolled Keywords: Pseudomonas aeruginosa PA0305. acyl-homoserine lactone acylase, elastase. pyocyanin
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Eko Setiawan 194014
Date Deposited: 25 Sep 2014 02:32
Last Modified: 05 Nov 2020 07:49

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