Papaioannou, Evelina and Wahjudi, Mariana and Jimenez, Pol Nadal and Koch, Gudrun and Setroikromo, Rita and Quax, Wim J. (2009) Quorum-Quenching Acylase Reduces the Virulence of Pseudomonas aeruginosa in a Caenorhabditis elegans Infection Model. Antimicrobial Agents and Chemotherapy, 53 (11). pp. 4891-4897.
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Abstract
The Pseudomonas aeruginosa PAO1 gene pvdQ encodes an acyl-homoserine lactone (AHL) acylase capable of degrading N-(3-oxododecanoyl)-l-homoserine lactone by cleaving the AHL amide. PvdQ has been proven to function as a quorum quencher in vitro in a number of phenotypic assays. To address the question of whether PvdQ also shows quorum-quenching properties in vivo, an infection model based on the nematode Caenorhabditis elegans was explored. In a fast-acting paralysis assay, strain PAO1(pMEpvdQ), which overproduces PvdQ, was shown to be less virulent than the wild-type strain. More than 75% of the nematodes exposed to PAO1(pMEpvdQ) survived and continued to grow when using this strain as a food source. Interestingly, in a slow-killing assay monitoring the survival of the nematodes throughout a 4-day course, strain PAO1-ΔpvdQ was shown to be more virulent than the wild-type strain, confirming the role of PvdQ as a virulence-reducing agent. It was observed that larval stage 1 (L1) to L3-stage larvae benefit much more from protection by PvdQ than L4 worms. Finally, purified PvdQ protein was added to C. elegans worms infected with wild-type PAO1, and this resulted in reduced pathogenicity and increased the life span of the nematodes. From our observations we can conclude that PvdQ might be a strong candidate for antibacterial therapy against Pseudomonas infections.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Eko Setiawan 194014 |
| Date Deposited: | 26 Sep 2014 02:39 |
| Last Modified: | 24 Mar 2021 14:55 |
| URI: | http://repository.ubaya.ac.id/id/eprint/20920 |
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