Elimination of Chrysanthemum stunt viroid (CSVd) from Meristem Tip Culture Combined with Prolonged Cold Treatment

Savitri, Wina Dian and Park, Kyung Il and Jeon , Su Min and Han, Jeung-Sul and Kim , Chang Kil (2013) Elimination of Chrysanthemum stunt viroid (CSVd) from Meristem Tip Culture Combined with Prolonged Cold Treatment. Horticulture, Environment and Biotechnology, 54 (2). pp. 177-182. ISSN 2211-3460

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Official URL: http://link.springer.com/article/10.1007/s13580-01...

Abstract

Chrysanthemum production in Korea has recently been greatly affected by the spread of Chrysanthemum stunt viroid (CSVd) infection, necessitating the use of CSVd-free stocks to ensure successful chrysanthemum cultivation. We investigated the effects of low temperature (4°C), antiviral chemicals (ribavirin and amantadine) and a combination of these treatments on CSVd elimination by meristem tip cultures using plantlets that originated from CSVd-infected chrysanthemum ‘Ency’. Neither antiviral agents led to CSVd elimination, despite the suppression of meristem tip growth in a concentration dependent manner. However, the CSVd elimination rate increased up to 42.8% when meristem tips were excised after storage at 4°C for two months. The most effective results were obtained from a combination of low temperature for three months at 4°C followed by meristem tip culture on media containing 50 and 100 mgL-1 ribavirin. These results suggest that antiviral agents can also be useful for CSVd elimination if their treatment is combined with prolonged periods of low temperature. This is the first report of eradication of viroids from spray type chrysanthemum bred in Korea.

Item Type: Article
Uncontrolled Keywords: amantadine, CSVd-free, low temperature, nested PCR, ribavirin
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: WINA DIAN SAVITRI
Date Deposited: 21 Nov 2014 04:08
Last Modified: 25 Nov 2014 02:11
URI: http://repository.ubaya.ac.id/id/eprint/21536

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