Elimination of Chrysanthemum stunt viroid (CSVd) from an Viroid infected Chrysanthemum through Shoot Tip Culture

Jeon, Su-Min and Savitri, Wina Dian and Park, Kyung-Il and Chung, Mi-Young and Kim, Chang-Kil (2012) Elimination of Chrysanthemum stunt viroid (CSVd) from an Viroid infected Chrysanthemum through Shoot Tip Culture. Flower Research Journal, 20 (4). pp. 218-222. ISSN 2287-772X

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Abstract

As the increase of chrysanthemum demand on chrysanthemum increases in Korea, the production of high quality chrysanthemum is needed. Chrysanthemum stunt viroid (CSVd) is one of the important viroid, which infects chrysanthemum and induces diseases that affects the decrease of quality and yield. To solve this problem, we used different size of meristem of chrysanthemum ‘Ency’ for shoot tip culture and also that of combined with heat treatment at 37οC. The efficiency of CSVd elimination was influenced by the size of shoot tip. The small-sized of meristems with 1 or 2 leaf primodia were regenerated into the highest number of CSVd-free plantlets. By RT-PCR, the 214-bp band corresponding to CSVd was not detected in 22.2% of the total number of tested regenerants from shoot tips with 2 leaf primordia. While, shoot tip culture combined with heat treatment of one-month-old in vitro shoots was not effective for CSVd-elimination. The CSVd-free plants grew more vigorously than CSVd-infected plants in the greenhouse.

Item Type: Article
Uncontrolled Keywords: CSVd-free plants, heat treatment, RT-PCR
Subjects: S Agriculture > SB Plant culture
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: WINA DIAN SAVITRI
Date Deposited: 21 Nov 2014 04:00
Last Modified: 25 Nov 2014 02:05
URI: http://repository.ubaya.ac.id/id/eprint/21539

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