Biohydrogen Production from Cane Molasses by Mutatted Enterobacter Aerogenes ADH43 in Continous Stirred Tank Reactor System with Varying Dilution Rate and Its Application into Fuel Cell for Generating Electricity.

Liasari, Yusnita (2011) Biohydrogen Production from Cane Molasses by Mutatted Enterobacter Aerogenes ADH43 in Continous Stirred Tank Reactor System with Varying Dilution Rate and Its Application into Fuel Cell for Generating Electricity. In: Bali International Seminar on Science and Technology (Bisstech 2011), 22-23 July 2011, Chemistry Departement, Udayana University.

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Abstract

Hydrogen gas (h2)produced by microorganism (biohydrogen) is acknowledged to be a clean and renewable energy resource for the future because its production does not require a large amount of energy and no COx, NOx and S0x emission is produced. Besides, the oxidation process of biohydrogen produce water and high energy (122kJ/g)without waste products, unlike fossil fuel. The aim of this research is to investigate the effect of dilutionrate tp biohydrogen production in continous stirred tank reactor (CSTR) system. The biohydrogen that was produced was directly inserted into a fuel cell to generate electricity. The substrate used in this research was cane molasses. The mutated bacteria used was Enterobacter aerogenes ADH41 that was a mutant bacteria having a inhibited metabolic pathway for acid production. Facultative anaerobe fermentation was conducted into stirred tank reactor using continuous system at 37c of temperature and 40rpm of agitation speed, dilution rate of (D) 0,15;0,3; 045; 0,60(h1).Biohydrogen produced was assesed after inserting it into a fuel cell to generate electricity and measuring voltages produced. The result showed that the highest H2 production was achieved at continuous system resulted in 0,30h1 of optimum dilution rate. The maximum H2 volume of 9,761 H2/l sugar, the yield of 1,84 H2/mol sugar, and the flow rate of 114,66 ml H2/minute were obtained. Furthermore, colony cont of 9,81 log cfu/ml, PH of 5,73, maximum electrical current of 0,38 A, electrical power of 2.20 watt, and electrical voltage of 5,75 volt after given resistance using LED of 25 ohm were also reached.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: H2 production, Enterobacter aerogenes, molasses, CSTR and Fuel Cell
Subjects: T Technology > TP Chemical technology
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Adpesdam Ubaya
Date Deposited: 21 Mar 2012 08:02
Last Modified: 21 Mar 2012 08:02
URI: http://repository.ubaya.ac.id/id/eprint/242

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