The Character of Biogas Fermentation on Simple Sugars by Enterobacter ludwigii Mutants

Wahjudi, Mariana and Macciano, Bryant Roossel and Rangan, Junus and Goeltom, Mangihot Tua (2015) The Character of Biogas Fermentation on Simple Sugars by Enterobacter ludwigii Mutants. Proceeding 1st International Seminar on Natural Resources Biotechnology : From Local to Global. ISSN 2460-8238

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Bio-hydrogen is one of prom1s1ng alternative energy nowadays. Pure-wild type bacteria commonly produce biohydrogen in a low level. In this research, we aim to carry on transposon mutagenesis of Enterobacter /udwigii, the isolate obtained from sediments of Kalimas River Surabaya, to produce more biohydrogen. The second aim was to characterize the properties of wild type and its mutants in biogas fermentation growing on simple sugars. Fermentations were performed anaerobically at 37°C and shaken at 200 rpm under dark condition. There were two target conjugant obtained, mutant A24-16 and 824-37. The E. ludwigii wild type, mutant A24-16 and 824-37 grew on M9 minimal medium containing glucose, xylose or arabinose without any differences between strains. All strains grew faster on glucose and arabinose compare to growth on xylose. Biogas was appeared since late logarithmic phase for glucose and arabinose or early stationary phase for xylose. The mutant A24-16 and 824-37 produced more biogas on all these sugars compared to its wild type. At the end of fermentation, sugars content of the cultures were diminished, which were determined as total reducing sugars. The pHs were decreased. It gave impressions that the sugars were consumed. The biogas produced during fermentations contained hydrogen. Ethanol and acetate were detected in the cultures at the end of fermentation. It can be concluded that the transposon mutagenesis of E. ludwigii generated two mutants, A24-16 and 824-37. The wild type and both mutants' cells could grow and produce biohydrogen on glucose, xylose or arabinose substrates in M9 minimal medium

Item Type: Article
Uncontrolled Keywords: Enterobacter ludwigii, mutant A24-16 and 824-37, biohydrogen, glucose, xylose, arabinose
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 23 Sep 2015 02:46
Last Modified: 23 Sep 2015 02:46

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