The Role and Efficiency of Ammonium Sulphate Precipitation in Purification Process of Papain Crude Extract

Purwanto, Maria Goretti Marianti (2016) The Role and Efficiency of Ammonium Sulphate Precipitation in Purification Process of Papain Crude Extract. Procedia Chemistry, 18. pp. 127-131.

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

It has been common to do fractionation (for example using ammonium sulphate as a precipitating agent) before doing a more sophisticated method for purification of a protein. The logic behind this is easy to understand, but in fact, the precipitation step often causes severe loss in yield and activity of the protein, making the whole purification effort too costly. In this work we evaluated the specific activity (thus, purification factor) and total activity (yield) during the purification process of papain from a crude extract using ion exchange chromatography (IEC), with and without prior fractionation using ammonium sulphate. Detail assays in each step were recorded and SDS-PAGE was also done to reveal the protein profile of the purification products

Item Type: Article
Uncontrolled Keywords: Ammonium sulphate; Precipitation; Purification
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 16 Feb 2016 10:16
Last Modified: 16 Feb 2016 10:16
URI: http://repository.ubaya.ac.id/id/eprint/26727

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