Development of Random Amplified Polymorphic DNA Method for Identity Assessment of Piper betle L., as a Starting Material for Herbal Medicine Product

Yunita, Oeke (2010) Development of Random Amplified Polymorphic DNA Method for Identity Assessment of Piper betle L., as a Starting Material for Herbal Medicine Product. Project Report. Indonesian Toray Science Foundation.

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Abstract

Development of RAPD method had been performed for DNA polymorphisms among many varieties and cultivars of Piper betle L. The source of DNA template is not from the DNA genome, but from the DNA fragment which had been resulted from amplification of DNA genome by Internal Transcribed Spacer (ITS) primer for Piper sp. The purpose of this research is mainly to develop the method for mapping the DNA fingerprint of herbal medicine (betelvine, Piper betle L.) using ITS region of betelvine nrDNA as DNA template by Random Amplified Polymorphic DNA, at locality levels. Therefore the developed method will be used for discriminating several cultivars of betelvine at Surabaya, Purwodadi and Batu. ST and SS had shown the similar bands than SB, this phenomenon was supported with the similar morphological conditions of ST and SS. P and B also had shown the similar bands each other but they had different DNA patterns if compared with ST, SS and SB.

Item Type: Monograph (Project Report)
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Oeke Yunita, S.Si, M.Si. Apt (1161)
Date Deposited: 23 Mar 2016 02:24
Last Modified: 23 Mar 2016 02:24
URI: http://repository.ubaya.ac.id/id/eprint/27158

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