Uji Daya Hambat Kitosan Hasil Transformasi Kitin dari Kulit Udang Windu (Penaeus monodon Fabricus) Terhadap Pertumbuhan Aspergillus flavus

-, Christinataliawati (2007) Uji Daya Hambat Kitosan Hasil Transformasi Kitin dari Kulit Udang Windu (Penaeus monodon Fabricus) Terhadap Pertumbuhan Aspergillus flavus. [Undergraduate thesis]

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Official URL: http://digilib.ubaya.ac.id/pustaka.php/149712

Abstract

Isolasi kitin dari kulit udang windu (Penaeus monodon Fabricus) dilakukan melalui tiga tahap utama ( deproteinasi, demineralisasi dan depigmentasi) dan didapatkan kitin sebesar 20%-22%. Kitin selanjutnya ditransformasi menjadi kitosan dan didapat hasil 63%-69%. Hasil isolasi dan transformasi diidentifikasi dengan uji kelarutan dan spektrofotometri inframerah. Hasil transformasi selanjutnya diuji daya hambatnya terhadap pertumbuhanjamur Aspergillusjlavus. Metode yang digunakan pada penelitian ini adalah metode difusi agar dengan menggunakan cylinder cup. Daya hambat diukur berdasarkan diameter hambatan pertumbuhan mikroba. Hasil penelitian menunjukkan bahwa kitosan dalam asam asetat 0,75% dengan konsentrasi 1%, 0,5%, 0,1% dan 0,01% tidak memberikan hambatan terhadap Aspergillus jlavus. Kata !cunei: Kitin, Kitosan, Udang Windu, Penaeus monodon, daya hambat, cylinder cup, Aspergillus jlavus ABSTRACT Chitin isolation from tiger prawn (Penaeus monodon Fabricus) shell is conducted through three main phases (deproteinization, demineralization and depigmentation) and 20%-22% chitin was obtained. The chitin was transformed into chitosan and the result was 63%-69%. The isolation and transformation results were identified with solubility test and infrared (IR) spectrophotometry. The inhibitory activity of the transformation result is then tested against the growth of Aspergillus jlavus fungus. The method used in this research is diffusion method in order to use cylinder cup. The inhibitory activity is measured by the diameter of microbe growth inhibition The result shows that chitosan in acetate acid of 0, 75% with concentration of l %, 0, 5%, 0, 1% and 0, 01% does not give the inhibition against Aspergillus jlavus.

Item Type: Undergraduate thesis
Uncontrolled Keywords: Chitin, chitosan, tiger prawn, Penaeus monodon, inhibitory activity, cylinder cup, Aspergillus jlavus
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Jumagi 197012
Date Deposited: 04 Apr 2016 07:32
Last Modified: 04 Apr 2016 07:32
URI: http://repository.ubaya.ac.id/id/eprint/27315

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