Production and Characterization of Chitinases from Thermophilic Bacteria Isolated from Prataan Hot Spring, East Java

Chrisnasari, Ruth and Yasaputera, Steven and Christianto, Pauline and Santoso, Veronika lvone and Pantjajani, Tjandra (2016) Production and Characterization of Chitinases from Thermophilic Bacteria Isolated from Prataan Hot Spring, East Java. Journal of Mathematical and Fundamental Sciences, 48 (2). pp. 149-163.

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Abstract

Thermophilic bacteria producing chitinase were collected from Prataan hot spring, East Java. Indonesia and screened The isolated bacterium was analyzed using 16S rRNA gene sequencing analysis and identified as Paenibacillus sp. The molecular identification was confirmed through morphological and physiological analyses. The production of chitinase was conducted at various incubation times, temperatures, pH and concentrations of colloidal chitin. The optimum condition of the isolate to produce the highest chitinase was 0.9% (w/v) of colloidal chitin (pH 7.0) at 48 °C for 24 hours. The obtained chitinases were optimally active at 55 oc and pH 6.0-7.0. The chitinases were gradually purified by ammonium sulfate precipitation, Sephadex G-1 00 gel filtration, followed by DEAE-cellulose ion exchange chromatography (IEC). The purification method gave a purification factor of9.43 and a yield of2.68%. Two protein fractions were obtained from sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-P AGE) with molecular weights of 68 and82k.Da.

Item Type: Article
Uncontrolled Keywords: chitin; chitinase,· Paenibacillus sp.; Prataan hot spring; thermophilic bacteria
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 30 Sep 2016 06:11
Last Modified: 30 Sep 2016 06:11
URI: http://repository.ubaya.ac.id/id/eprint/28457

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