Immobilization of Chondroitin Sulfate A into Monolithic Epoxy Column for Chiral Separation

Ratih, Ratih and Asmari, Mufarreh and Öner, Selin and Deeb, Sami El (2017) Immobilization of Chondroitin Sulfate A into Monolithic Epoxy Column for Chiral Separation. In: HPLC 2017, 18-22 Juni 2017, Czech Republic.

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Chondroitin sulfate A was successfully immobilized into epoxy monolithic column at a concentration of 3% (w/v) in the presence of ethylene diamine. The epoxy group of monolithic column was first converted to aldehyde group by successive hydrolization and oxidation. A Schiff base reaction at pH 8.0 was used to attach the diamine-spacer to aldehyde group. The chondroitin sulfate A was introduced into the monolithic column by circulating the solution at a flow rate of 0.1 mL/min for 24 hours. The chondroitin sulfate A-immobilized epoxy column was evaluated for chiral separation of verapamil enantiomers under optimized HPLC conditions at a wavelength of 230 nm. As a mobile phase, 20 mM Na2HPO4 (pH 2.9) was used. A resolution (Rs) of about 1.5 was achieved for the separation of verapamil enantiomers. A good repeatability of the retention time at two concentration levels (n=8) with RSD < 1% was obtained. The linear responses of verapamil enantiomers were in the range of 1.0-3.0 ppm with R2 of about 0.994.

Item Type: Conference or Workshop Item (Poster)
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Eko Setiawan 194014
Date Deposited: 12 Jul 2017 02:32
Last Modified: 15 Dec 2023 05:01

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