THE ANTIOXIDANT COMPOUNDS IN METHANOL EXTRACT OF Glycine max L. Merr DETAM I AND II VARIETIES by KINETIC MACERATION METHOD

Yulia, Rika and Christyaningsih, Juliana and KRISTINA, ERNI MEI and SOEGIONO, NOFIE KUSUMAWATI (2016) THE ANTIOXIDANT COMPOUNDS IN METHANOL EXTRACT OF Glycine max L. Merr DETAM I AND II VARIETIES by KINETIC MACERATION METHOD. In: International Conference on Health Polytechnic Surabaya, 15-16 November 2016, Poltekkes Kemenkes Surabaya.

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Abstract

The study aims to identify the active compound of antioxidants in the methanol extract of black soybean (Glycine max L. Merr) Detam I and II varieties. The extract results from maceration kinetics was analyzed with GC-MS. Soybeans have been crushed then carried defatting using n-hexane solvent, the pulp obtained from the defatting then extracted using methanol 90%. Qualitative analysis was performed using TLC and GC-MS Shimadzu QP-2010 Model SE with a total time of analysis 20 and 80 minutes. The presence of compounds flavonols 3',4',5,7-OH, 3-O araglucoside and hexadecanoic acid, methyl ester; pentadecanoic acid, 14-methyl, -methyl ester; 9,12-Octadecadienoic acid (Z, Z) - methyl ester and methyl 10-trans, 12-cis-octadecadienoate. TLC results condensed methanol extract of black soybean (Glycine max L. Merr) Detam II varieties also contain flavonoids, but the GC-MS’s profile showed no detectable flavonoids but there are several antioxidant compounds i.e Octanoic acid; Methyl 10-trans, 12-cis-octadecadienoate; Hexadecanoic acid; and 9,12-Octadecadienoic acid (Z, Z).

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Detam I and II varieties of Glycine max L. Merr, Antioxidants, TLC and GC-MS
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Eko Setiawan 194014
Date Deposited: 25 Aug 2017 06:39
Last Modified: 25 Aug 2017 06:39
URI: http://repository.ubaya.ac.id/id/eprint/30617

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