Green Pigment Production by Shoot Cultures of Sauropus androgynus

Yunita, Oeke and Krisnawan, Alfian Hendra and Wijayanto, Devi Veronica and Hapsari, Aulia Dwi and Sari, Tri Muji Lestari Wahyu Wilang (2016) Green Pigment Production by Shoot Cultures of Sauropus androgynus. In: International Conference on Natural Resources and Life Sciences (NRLS), 21-22-Oktober-2016, Universitas Surabaya. (Unpublished)

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The dark green leaves of Sauropus androgynus (Euphorbiaceae) have various nutritive values and are commonly used for human consumption in Southeast Asia. Shoot cultures of this plant were established by adding various concentrations of kinetin (Kn) and 6-benzyl adenine (BA). The best results were recorded when Kn 0.1 ppm was used with BA 1 ppm (BA1Kn0,1). Spectrophotometric analysis showed two peaks of green pigment in shoot cultures, Y pigment (λmax = 663.6 - 663.8 nm, absorbance 0.1111) and Z pigment (λmax = 611.3 - 613.9 nm, absorbance 0.0390). Thin Layer Chromatography (TLC) analysis method showed two green spots (Rf Y = 0.3125 and Rf Z = 0.25) of shoot cultures on medium suplemented with BA1Kn0,1 during 10 days. Pigment profiles of shoot cultures were similar to their corresponding mother plants. Random Amplified Polymorphic DNA (RAPD), used as a preliminary technique to evaluate genetic similarity of the shoot cultures and their corresponding mother plants, showed five similar DNA banding patterns to their leaves, ranging from 271-765 bp.

Item Type: Conference or Workshop Item (Poster)
Uncontrolled Keywords: Sauropus androgynus, green pigment, shoot cultures, Spectrophotometry, Thin Layer Chromatography, RAPD
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Eko Setiawan 194014
Date Deposited: 16 Oct 2017 01:41
Last Modified: 02 Sep 2021 02:49

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