SKRINING TRANSFORMASI GENETIK TANAMAN TEBU (Saccharum spp. hybrids) DENGAN PERANT ARA Agrohacterium tumefaciens

Hardjo, Popy Hartatie and Darmanto, Win and Sugiharto, Bambang (2014) SKRINING TRANSFORMASI GENETIK TANAMAN TEBU (Saccharum spp. hybrids) DENGAN PERANT ARA Agrohacterium tumefaciens. Jurnal Ilmiah Sains & Teknologi, 7 (2). pp. 1-6. ISSN 0216-1540

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Abstract

The plants characteristics after transforming intended to show that the success of transforming, therefore, screening methods of transformed population is needed. Selection methods had been done 5 cycles for 3 weeks at the medium of hygromycin. This methods was preliminary methods which could be used to identify the transformed plants which carried SoSUTI gene, which was transformed in the plants because that gene was bound together with hpt gene which expressed phosphotransferase enzyme for endurance towards hygromycin antibiotic. The hygromycin concentration used to do transformed screening was determined by growing non-transformant sugarcane shoot at the MS medium with several hygromycin concentrations (0-40 mg/l). Hygromycin concentration, which was capable to kill the sugarcane shoot, was used further for selecting the result of Agrobacterium tumefacieris GV 3010 mediated transformed genes in the sugarcane shoot. The 15 mg/1 hygromycin concentration was used to do transformed screening for 2 cycles of selection, and it would be done further on 3, 4 and 5 cycles of selection using hygromycin 20, 25 and 30 mg/l. From the result of 500 explants shoot transformation, 41 shoots was able to grow at the selection medium with the transformation effective of 8.2 %.

Item Type: Article
Uncontrolled Keywords: screening, transformant, sugarcane, Agrobacterium tumefaciens
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 01 Nov 2017 05:02
Last Modified: 02 Nov 2017 03:01
URI: http://repository.ubaya.ac.id/id/eprint/31042

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