Cupriavidus yeoncheonense sp. nov., isolated from soil of ginseng

Singh, Priyanka and Kim, Yeon-Ju and Nguyen, Ngoc-Lan and Hoang, Van-An and Sukweenadhi, Johan and Farh, Mohamed El-Agamy and Yang, Deok-Chun (2015) Cupriavidus yeoncheonense sp. nov., isolated from soil of ginseng. Antonie van Leeuwenhoek, 107 (3). pp. 749-758. ISSN 0003-6072 (print), 1572-9699 (online)

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A novel bacterial strain, DCY86T (=KCTC 42053T = JCM 19890T) was isolated from soil of a ginseng field in Yeoncheon province (38°04′00″N 126°57′00″E), Republic of Korea using a serial dilution method. Strain DCY86T was observed to be Gram-stain negative, strictly aerobic, to grow optimally at 25–30 °C, at pH 7–7.5 and on tryptic soya agar medium. The cells were found to be sensitive to ceftazidine and tetracycline. Based on 16S rRNA gene sequence comparisons, strain DCY86T was found to be most closely related to Cupriavidus basilensis LMG 18990T (98.48 %), Cupriavidus numazensis LMG 26411T (98.34 %), Cupriavidus pinatabonesis KCTC 22125T (98.34 %) and Cupriavidus laharis KCTC 22126T (98.00 %). The G+C content was determined to be 64.23 mol %. The only isoprenoid quinone detected in strain DCY86T was ubiquinone Q-8. The major polar lipids were identified as diphosphatidylglycerol, phosphtidylethanolamine, phosphatidylglycerol, unidentified aminophosphoglycolipids and unidentified phospholipids. The major fatty acids were identified as C16:0 summed feature 3 (C16:1 ω7c/ω6c and/or iso-C15 : 0 2-OH) and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). These data support the affiliation of strain DCY86T to the genus Cupriavidus. Strain DCY86T was also found to be able to solubilize phosphate and produce siderophores. The results of physiological and biochemical tests enabled strain DCY86T to be differentiated genotypically and phenotypically from the recognized species of the genus Cupriaividus. Therefore, the novel isolate can be considered to represent a novel species, for which the name Cupriavidus yeoncheonense sp. nov. is proposed here. The type strain is DCY86T (=KCTC 42053T = JCM 19890T).

Item Type: Article
Uncontrolled Keywords: Cupriavidus yeoncheonense , Proteobacteria , Ginseng,Polyphasic taxonomy
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Technobiology > Department of Biology
Date Deposited: 21 Nov 2017 08:57
Last Modified: 22 Nov 2017 06:56

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