Novel Scanometric Assay for Charantin in Bitter Melon (Momordica charantia) Extract Based on Immobilized Silver Nitrate and Methylene Blue as Colorimetric Paper

Afthoni, Muhammad Hilmi and Wahjudi, Mariana and Kuswandi, Bambang (2018) Novel Scanometric Assay for Charantin in Bitter Melon (Momordica charantia) Extract Based on Immobilized Silver Nitrate and Methylene Blue as Colorimetric Paper. Journal of Food Chemistry & Nanotechnology, 4 (2). pp. 24-32.

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Official URL / DOI: https://doi.org/10.17756/jfcn.2018-054

Abstract

Bitter melon (Momordica charantia) contains charantin that responsible for the antidiabetic activity. Therefore, it is important to determine charantin in the extracts for the purposed of its formulation or quality control. Herein, we proposed a novel scanometric assay for charantin determination in the bitter melon extracts based on immobilized silver nitrate and methylene blue as a colorimetric paper. The presence of charantin can be detected simply via the color change from blue to green and can be quantified using a scanner via the ImageJ software. The proposed charantin sensor has a response time of 8 mins with the linear range of 300 to 1000 μg/mL and the detection limit was 216.25 μg/mL. The sensor has good reproducibility (RSD = 2.86%) with the stability up-to 5 months. The sensor was applied to determine charantin in the sample extracts, and the result shown in a good agreement with TLC method.

Item Type: Article
Uncontrolled Keywords: Colorimetric sensor, Charantin, Silver nitrate, Methylene blue, Bitter melon
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Eko Setiawan 194014
Date Deposited: 21 Nov 2018 03:47
Last Modified: 01 Apr 2020 07:40
URI: http://repository.ubaya.ac.id/id/eprint/33902

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