Thermophoresis for characterizing biomolecular interaction

Asmari, Mufarreh and ., Ratih and Alhazmi, Hassan A. and El Deeb, Sami (2018) Thermophoresis for characterizing biomolecular interaction. Methods, 146. 107-119. ISSN 1046-2023

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The study of biomolecular interactions is crucial to get more insight into the biological system. The interactions of protein-protein, protein-nucleic acids, protein-sugars, nucleic acid-nucleic acids and proteinsmall molecules are supporting therapeutics and technological developments. Recently, the development in a large number of analytical techniques for characterizing biomolecular interactions reflect the promising research investments in this field. In this review, microscale thermophoresis technology (MST) is presented as an analytical technique for characterizing biomolecular interactions. Recent years have seen much progress and several applications established. MST is a powerful technique in quantitation of binding events based on the movement of molecules in microscopic temperature gradient. Simplicity, free solutions analysis, low sample volume, short analysis time, and immobilization free are the MST advantages over other competitive techniques. A wide range of studies in biomolecular interactions have been successfully carried out using MST, which tend to the versatility of the technique to use in screening binding events in order to save time, cost and obtained high data quality

Item Type: Article
Uncontrolled Keywords: Microscale thermophoresis, Binding interactions,Binding constant, Hill equation
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Ester Sri W. 196039
Date Deposited: 13 Feb 2019 02:28
Last Modified: 13 Feb 2019 03:23

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