Menthol from the stem and leaf in-vitro Mentha piperita Linn.

Wijaya, B.K. and Hardjo, Popy Hartatie and Putra, Sulistyo Emantoko Dwi (2019) Menthol from the stem and leaf in-vitro Mentha piperita Linn. In: The 2nd International Conference on Natural Resources and Life Sciences (NRLS-2018), 23-24 August 2018, Ibis Styles Hotel, Surabaya.

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The need for menthol in Indonesia is increasing annually, but it is not followed by increases the availability in the country, so the number of imports is increasing each year. Therefore, in this research conducted a study using plant tissue culture techniques to produce secondary metabolite especially menthol using shoot multiplication. A single node of Mentha piperita L. was inoculated on three kinds of growth medium, which are MS + 0.1 mg L–1 NAA + 0.1 mg L–1 BA, MS + 0.5 mg L–1 BA, and MS + 2 mg L–1 BA. Based on fresh weight and morphological observation, MS + 0.1 mg L–1 NAA + 0.1 mg L–1 BA was the best growth medium. Based on the previous experiment, the study was aiming to continue observing the concentration of menthol found in M. piperita shoot. Stems and leaves of M. piperita were dried in room temperature, the dried stems and leaves were extracted using steam distillation method with ratio 0.3 g of dried stems and leaves in 25 mL aquadest. The crude extract was analyzed by gas chromatography (HP 6890) using INNOWAX 19095N-123 column. The results showed that 2 wk was the best amount of time to obtain the highest concentration of menthol specifically (1 218.5 ± 47.1) mg L–1 (yield = 9.748 %).

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Gas chromatography, menthol, shoot multiplication, steam distillation
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Ester Sri W. 196039
Date Deposited: 31 Jul 2019 02:49
Last Modified: 25 Apr 2022 07:51

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