Saadah, Ira Nailas and Kristanti, Alfinda Novi and Hardjo, Popy Hartatie and Manuhara, Yosephine Sri Wulan (2019) Shoots Culture of Gynura procumbens (Lour.) Merr. in Balloon-Type Bubble-bioreactor Influenced by Sucrose Concentration and Inoculums Density. Asian Journal of Plant Sciences, 18 (2). pp. 85-90. ISSN 1682-3974
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Abstract
Background and objectives: Gynura procumbens has been widely used as a traditional medicine in Indonesia. Micropropagation of this plant was important to fulfill the need of pharmaceutical industry. The aims of this study were to increasing the number of G. procumbens shoots in balloon-type bubble bioreactor influenced by sucrose concentration and inoculums density. Materials and Methods: Nodal segments of G. procumbens were used as explants which were grown in balloon type bubble bioreactor in MS liquid medium supplemented with 2 mg LG1 IAA and 4 mg LG1 BAP. Shoot culture were conducted under treatment of various concentration of sucrose (10, 30 and 50 g LG1) and various inoculums density (5, 10 and 15 explants). Results: The highest multiplication of shoots and growth index were obtained at 50 g LG1 sucrose treatment and 5 explants as initial inoculums; there are 9.36 shoots/explants and 13.8 shoots/explants, respectively. Conclusion: The study found that G. procumbens shoots cultured in balloon-type bubble bioreactors increased shoots multiplication significantly.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Balloon-type, bubble bioreactor, Gynura procumbens , inoculums density, sucrose, shoot culture |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Faculty of Technobiology > Department of Biology |
| Depositing User: | Ester Sri W. 196039 |
| Date Deposited: | 02 Mar 2020 08:07 |
| Last Modified: | 09 Feb 2021 03:06 |
| URI: | http://repository.ubaya.ac.id/id/eprint/37518 |
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