Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture

Hardjo, Popy Hartatie and Krisnawan, Alfian Hendra (2020) Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture. Media Pharmaceutica Indonesiana, 3 (1). pp. 19-26. ISSN -p 2527-6298; e-ISSN 2527-9017

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Official URL / DOI: https://doi.org/10.24123/mpi.v3i1.2836

Abstract

The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L Benomyl combined with NaOCl decreased the contamination of explants, and achieved 20% axenic culture.

Item Type: Article
Uncontrolled Keywords: rhizome bud; red ginger; explant sterilization methods
Subjects: R Medicine > RS Pharmacy and materia medica
T Technology > T Technology (General)
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Ester Sri W. 196039
Date Deposited: 12 Aug 2020 08:27
Last Modified: 12 Aug 2020 08:27
URI: http://repository.ubaya.ac.id/id/eprint/38001

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