Standardization of a Crude Drug Moringa oleifera Leaf from Africa, India and Local (Indonesian) which Cultivated in Bojonegoro Indonesia

Jayani, Nikmatul Ikhrom Eka and Kartini, Kartini and Putri, Lintang Karina (2020) Standardization of a Crude Drug Moringa oleifera Leaf from Africa, India and Local (Indonesian) which Cultivated in Bojonegoro Indonesia. INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH, 12 (1). pp. 660-668. ISSN 0975-2366

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Abstract

Purpose: The research was to determine specific parameters (macroscopy and microscopy) and non-specific parameters (loss on drying, total ash content, acid insoluble ash content, water-soluble extractive matter, ethanol-soluble extractive matter), heavy metal contaminant (Pb, Hg, As, Cu) and microbial contaminant (Total Plate Count and Total Yeast Mold). Research also determined a total flavonoid of M. oleifera leaf. Methodology: The method carried out according to the procedure stated in the Materia Medika Indonesia 5 th edition. Result test compared to the standard stated in Indonesian Herbal Pharmacopoeia 2 nd edition. Methods to detection Contaminants were referred to WHO guidelines for assessing the quality of herbal medicines. The determination of total flavonoid was performed by spectroscopic method. Results: Crude drug of M. oleifera leaf meet the specific parameters (macroscopy and microscopy). Nonspecific parameters of Africa, India, Local M. oleifera leaf shows that loss on drying (8.06 ± 0.03; 8.89 ± 0.31; 7.56 ± 0.17) %, total ash content (8.64 ± 0.43; 10.64 ± 0.90; 15.31 ± 0.87) %, acid insoluble ash content (0.56 ± 0.08; 0.35 ± 0.01; 0.36 ± 0.06)%, water-soluble extractive matter (21.38 ± 1.39; 30.12 ± 2.06; 12.68 ± 1.12) %, ethanol-soluble extractive matter (39.37 ± 1.51; 27.74 ± 2.44; 27.09 ± 1.43)%, contaminant test including heavy metal contaminant shows that Pb, Cd, As and Hg were not to be detected. Microbial contaminant (Total Plate Count and Total Yeast Mold) under limits of WHO standart. Total flavonoid content of Africa, India, Local M. oleifera leaf were 8.12 ± 0.52 mg/ 100 mg QE; 10.69 ± 0.15 mg/ 100 mg QE dan 13.08 ± 0.08 mg/ 100 mg QE respectively. Conclusion: Moringa Leaves meet specific parameters (macroscopy and microscopy test). Non-specific parameter tests of Africa, India, Local Moringa Leaf shows that loss on drying, acid insoluble ash content, water-soluble extractive matter, ethanol-soluble extractive matter, contaminant test including heavy metal contaminant shows that (Pb, Cd, As and Hg) and microbial contaminant (Total Plate Count and Total Yeast Mold) meet the standard required, only total ash content did not meet the standard. Total Flavonoid content shows that the extract of M. oleifera from local (Indonesia) variety is higher than others. Key words: M. oleifera leaf, Standardization, Crude Drug

Item Type:	Article
Additional Information:	The authors are thankful to LPPM UBAYA, Indonesia for providing internal research grants in 2019(No. 008/SPLit/LPPM-01/Int/FF/III/2019) to support this research.
Uncontrolled Keywords:	M. oleifera leaf, Standardization, Crude Drug
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	NIKMATUL IKHROM EKA JAYANI
Date Deposited:	17 Nov 2020 09:04
Last Modified:	24 Sep 2021 07:33
URI:	http://repository.ubaya.ac.id/id/eprint/38508

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