Sukweenadhi, Johan and Kim, Yu-Jin and Yang, Deok-Chun (2020) Transcription Pattern of Catalase Gene from Gynostemma pentaphyllum (Thunb.) Makino during Various Abiotic Stresses. In: International Conference on Natural Resources and Life Sciences (NRLS-2016), 20–21 October 2016, Surabaya.
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Abstract
Catalase (CAT) is a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species. A CAT cDNA was previously isolated and characterized from 3-month-old hydroponically cultured Gynostemma pentaphyllum(Thunb.) Makino plants. The ORF is 1 479 bp with a deduced amino acid sequence of 492 residues. CAT from G. pentaphyllum has a molecular mass of 56.97 kDa with an isoelectric point (pI) of 6.95. The temporal expression analysis of leaf samples demonstrated that GpCAT expression could be up-regulated by various environmental stresses such as jasmonic acid electro, oxidative, salt, heavy metal, chilling and heat stress in a certain time period. A three-dimensional structural model of G. pentaphyllum based on its GpCAT cDNA sequence. The temporal expression pattern suggests that the GpCAT could play a role in the molecular defense response of G. pentaphyllum to abiotic stresses.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Uncontrolled Keywords: | Abiotic stress; Catalase; Gene expression; Gynostemma pentaphyllum(Thunb.) Makino |
| Subjects: | T Technology > T Technology (General) |
| Divisions: | Faculty of Technobiology > Department of Biology |
| Depositing User: | Ester Sri W. 196039 |
| Date Deposited: | 18 Nov 2020 03:21 |
| Last Modified: | 19 Oct 2021 07:57 |
| URI: | http://repository.ubaya.ac.id/id/eprint/38514 |
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