Critical Aspects to Produce Low-Cost Protein Molecular Weight Marker : A Review

Gunawan, Leonardo Tejo and Purwanto, Maria Goretti Marianti (2020) Critical Aspects to Produce Low-Cost Protein Molecular Weight Marker : A Review. Indonesian Journal of Biotechnology and Biodiversity, 4 (2). pp. 118-127. ISSN 2581-0014

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Abstract

Proteomic studies usually begin with characterizing protein profile, like the molecular weight of protein, which can be done by the SDS-PAGE technique followed by Western Blot. These methods need a standard protein called molecular weight marker (MWM). In this review, the important, basic aspects of either recombinant or native MWM production was discussed, including the type and effect of dyes can be used for preparing a prestained MWM. Moreover, buffer and polyols used in the formulation can also affect the quality and stability. Also, another adjuvant may be needed to increase the robustness of MWM to lower the risk of protease contamination that can breakdown the protein inside the MWM during storage. Understanding those critical aspects will help to produce/formulate a good quality of MWM.

Item Type:	Article
Uncontrolled Keywords:	protein ladder, protein stability, electrophoresis
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Eko Setiawan 194014
Date Deposited:	07 Jan 2021 23:16
Last Modified:	25 May 2022 03:00
URI:	http://repository.ubaya.ac.id/id/eprint/38734

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