Immobilization of Chondroitin Sulfate A onto Monolithic Epoxy Silica Column as a New Chiral Stationary Phase for High-Performance Liquid Chromatographic Enantioseparation

Ratih, Ratih and Wätzig, Hermann and Azminah, Azminah and Asmari, Mufarreh and Peters, Benjamin and Deeb, Sami El (2021) Immobilization of Chondroitin Sulfate A onto Monolithic Epoxy Silica Column as a New Chiral Stationary Phase for High-Performance Liquid Chromatographic Enantioseparation. Pharmaceuticals, 14 (2). ISSN 1424-8247

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Official URL / DOI: https://doi.org/10.3390/ph14020098

Abstract

Chondroitin sulfate A was covalently immobilized onto a monolithic silica epoxy column involving a Schiff base formation in the presence of ethylenediamine as a spacer and evaluated in terms of its selectivity in enantioseparation. The obtained column was utilized as a chiral stationary phase in enantioseparation of amlodipine and verapamil using a mobile phase consisting of 50 mM phosphate buffer pH 3.5 and UV detection. Sample dilution by organic solvents (preferably 25% v/v acetonitrile-aqueous solution) was applied to achieve baseline enantioresolution (Rs > 3.0) of the individual drug models within 7 min, an excellent linearity (R2 = 0.999) and an interday repeatability of 1.1% to 1.8% RSD. The performance of the immobilized column for quantification of racemate in commercial tablets showed a recovery of 86–98% from tablet matrices. Computational modeling by molecular docking was employed to investigate the feasible complexes between enantiomers and the chiral selector. Keywords: amlodipine; chiral stationary phase; chondroitin sulfate A; enantioseparation; immobilization; monolithic column; Schiff base; verapamil

Item Type: Article
Uncontrolled Keywords: amlodipine; chiral stationary phase; chondroitin sulfate A; enantioseparation; immobilization; monolithic column; Schiff base; verapamil
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: AZMINAH 204031
Date Deposited: 08 Apr 2021 08:31
Last Modified: 08 Apr 2021 08:31
URI: http://repository.ubaya.ac.id/id/eprint/39192

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