Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production

Askitosari, Theresia Desy and Berger, Carola and Tiso, Till and Harnisch, Falk and Blank, Lars M. and Rosenbaum, Miriam A. (2020) Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production. Microorganisms, 8 (12). pp. 1959-1. ISSN 2076-2607

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Abstract

Sufficient supply of oxygen is amajor bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using Pseudomonas putida KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering the process. It is caused by the extensive bubbling needed to sustain the high respirative oxygen demand in the presence of the produced surfactants. One way to reduce the oxygen requirement is to enable the cells to use the anode of a bioelectrochemical system (BES) as an alternative sink for their metabolically derived electrons. We here used a P. putida KT2440 strain that interacts with the anode using mediated extracellular electron transfer via intrinsically produced phenazines, to performheterologous rhamnolipid production under oxygen limitation. The strain P. putida RL-PCA successfully produced 30.4 � 4.7mg/Lmono-rhamnolipids togetherwith 11.2 � 0.8mg/L of phenazine-1-carboxylic acid (PCA) in 500-mL benchtop BES reactors and 30.5 � 0.5 mg/L rhamnolipids accompanied by 25.7 � 8.0 mg/L PCA in electrode containing standard 1-L bioreactors. Hence, this study marks a first proof of concept to produce glycolipid surfactants in oxygen-limited BES with an industrially relevant strain.

Item Type:	Article
Uncontrolled Keywords:	Pseudomonas putida; rhamnolipid; bioelectrochemical system; phenazines; redox mediator; microbial electrosynthesis; electrobiotechnology; metabolic engineering
Subjects:	T Technology > T Technology (General)
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Ester Sri W. 196039
Date Deposited:	05 Aug 2021 04:24
Last Modified:	06 Aug 2021 09:00
URI:	http://repository.ubaya.ac.id/id/eprint/39947

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