Optimization of betaine-sorbitol natural deep eutectic solvent-based ultrasound-assisted extraction and pancreatic lipase inhibitory activity of chlorogenic acid and caffeine content from robusta green coffee beans

Ahmad, Islamudin and Syakfanaya, Adisya Miftah and Azminah, Azminah and Saputri, Fadina Chany and Mun'im, Abdul (2021) Optimization of betaine-sorbitol natural deep eutectic solvent-based ultrasound-assisted extraction and pancreatic lipase inhibitory activity of chlorogenic acid and caffeine content from robusta green coffee beans. Heliyon, 7 (8). pp. 1-9.

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Abstract

Natural deep eutectic solvent (NADES) is an alternative approach in natural product extraction with various advantages, including low toxicity, biodegradable, and suitable phytochemical compounds in a wide range of polarity. Chlorogenic acid (CGA) and caffeine, a well-known compound in the coffee bean, have various potential health benefits. This study aims to optimize the betaine–sorbitol NADES-based ultrasound-assisted extraction (UAE) method of CGA and caffeine from Robusta green coffee beans and determine the inhibitory activity of robusta green coffee beans extract of the betaine-sorbitol NADES-UAE from the optimum condition on pancreatic lipase in vitro and in silico. The betaine-sorbitol NADES-UAE factors as experimental design variable parameters include betaine-sorbitol ratio (0.5:1.2, 1.25:1.2, and 2:1.2 mol), extraction time (10, 35, and 60 min), and solid-liquid ratio (1:10, 1:20, and 1:30 g/mL). Response surface methodology and Box-Behnken Design were used to optimize the extraction process. The response surface was calculated by using CGA and caffeine content as response values. CGA and caffeine content was determined by High-Performance Liquid Chromatography. Whereas in vitro lipase inhibitory activity assay examined by spectrophotometric measurement and in silico molecular docking analysis on PDB ID: 1LPB. According to the results, the optimum conditions of the betaine-sorbitol NADES-UAE have obtained the betaine-sorbitol ratio of 1.25: 1.2 mol, solid-liquid ratio of 1:30 mg/mL, and 60 min extraction time. Furthermore, obtained Robusta green coffee extract from the optimum condition of the betaine-sorbitol NADES-UAE showed high potential to inhibit lipase activity with IC50 of 18.02 μg/ml, comparable with IC50 of standard CGA (11.90 μg/ml) and caffeine (15.59 μg/ml), where potential interaction of both standards was confirmed using molecular docking analysis. Our finding demonstrated the optimum condition of the betaine-sorbitol NADES-UAE method for CGA and caffeine extraction and the potential pancreatic lipase inhibition activity from the Robusta green coffee bean. Keywords CaffeineChlorogenic acidNatural deep eutectic solventPancreatic lipaseRobusta green coffee beansUltrasonic-assisted extraction

Item Type:	Article
Uncontrolled Keywords:	Caffeine; Chlorogenic acid; Natural deep eutectic solvent; Pancreatic lipase; Robusta green coffee beans; Ultrasonic-assisted extraction
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	AZMINAH 204031
Date Deposited:	16 Aug 2021 07:52
Last Modified:	16 Aug 2021 07:52
URI:	http://repository.ubaya.ac.id/id/eprint/40004

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