Ethyl Methane Sulfonate (EMS) Effect on Mutagenesis in Balinese Red Rice (Oryza sativa cv. Barak Cenana)

Artadana, Ida Bagus Made and Hardjo, Popy Hartatie and Purwanto, Maria Goretti Marianti and Pujiyanti, Aloysia Sri (2021) Ethyl Methane Sulfonate (EMS) Effect on Mutagenesis in Balinese Red Rice (Oryza sativa cv. Barak Cenana). Jurnal Biologi Tropis, 21 (3). pp. 698-705. ISSN 25497863

PDF Ida Bagus Made Artadana_Ethyl Methane Sulfonate (EMS) Effect .pdf Download (1MB)

Abstract

“Barak Cenana” is one of the local red rice (Oryza sativa) with high economic value due to its nutrient content. Some of the agronomic characters such as tall shoot, low number of tiller, and late harvesting are drawbacks to improving production. Ethyl Methyl Sulphonate (EMS) is a chemical mutagen widely used to improve rice characters by mutation breeding. The present research aims to investigate the EMS concentration suitable to induce mutation in Barak Cenana and observe the mutagenesis effect of EMS on some agronomic characters of Barak Cenana. For those purposes, mature seeds of Barak Cenana were treated with various concentrations of EMS (0.25, 0.5, 0.75, 1.0, and 1.1 %) for 24 hours. Seed germination, plant growth, some agronomic character related to productivity were compared to untreated rice. EMS reduced and delayed seed germination and inhibit shoot and root growth in early vegetative stage. The ability of EMS to reduce and delay seed germination and inhibit shoot and root growth were doses dependent. EMS concentration of more than 1.0% was lethal for Barak cenana and EMS concentration at 0.75% was able to reduce rice productivity by increasing seed sterility. EMS concentrations between 0.25 and 0.5% with 24 h of soaking are suitable for inducing mutagenesis in Barak Cenana. Additionally, some mutants with distinct morphology such as sterile plant, semi-dwarf, high tillering capacity, and high chlorophyll contents were produced in this research.

Item Type:	Article
Uncontrolled Keywords:	EMS; Mutagenesis; Agronomic; Germination; Productivity
Subjects:	Q Science > Q Science (General)
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Ide Bagus Made Arta D 2007004
Date Deposited:	17 Sep 2021 02:54
Last Modified:	17 Sep 2021 03:13
URI:	http://repository.ubaya.ac.id/id/eprint/40331

Actions (login required)

View Item