Gene Mutations in Mycobacterium tuberculosis Based on Pyrosequencing Assay and Its Role on Antituberculosis-drugs Resistance: A Literature Review

Raharjo, Dian Natasya and Agatha, Cindy Valencia and Wahjudi, Mariana and Sartika, Ratu Ayu Dewi and Andrajati, Retnosari (2021) Gene Mutations in Mycobacterium tuberculosis Based on Pyrosequencing Assay and Its Role on Antituberculosis-drugs Resistance: A Literature Review. In: The 1st International Conference on Pharmaceutical Sciences and Military Pharmacy (ICOPMAP), 3-4 September 2021, Bogor.

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Abstract

Background: Mycobacterium tuberculosis as the cause of tuberculosis disease is known to have some various genotype variations which can cause antibiotic resistance or cause the bacteria more susceptible to resistance than any other variation. Objective: to determine the mutation in the genes of Mycobacterium tuberculosis detected by pyrosequencing assay that could cause rifampicin and isoniazid resistance. Methods: A literature review is carried out on the included articles obtained from PubMed and Google Scholar database search results. The literature search used the PRISMA flowchart principle. Articles from the search result are checked for duplication and selected through predetermined inclusion and exclusion criteria, in which the journal's reputation is checked according to the Scopus index or SINTA and a critical review of each article is carried out. Selected articles will be analyzed using the narrative review method. Results: The search results using the PubMed and Google Scholar database are 725 articles. After going through duplication checks, selection according to inclusion and exclusion criteria, as well as checking journal reputation and critical studies, five articles will be analyzed to answer the research objective. In rifampicin resistance, many gene mutations occurred in rpoB Ser531Leu, while in isoniazid resistance mutations occurred in katG Ser315Thr and inhA 15 C-T. Conclusion: There are some mutations in Mycobacterium tuberculosis associated with antituberculosis-drug resistance that can be detected by pyrosequencing assay Keywords: Mycobacterium tuberculosis; drug resistance; pyrosequencing; gene mutation

Item Type:	Conference or Workshop Item (Speech)
Uncontrolled Keywords:	Mycobacterium tuberculosis; drug resistance; pyrosequencing; gene mutation
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	DIAN NATASYA R - 210004
Date Deposited:	29 Oct 2021 03:02
Last Modified:	27 Sep 2023 04:24
URI:	http://repository.ubaya.ac.id/id/eprint/40655

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