The Expression Change of MMP-8 and Collagen Type-2 Intracell in Lung Tissue Due to Electronic Smoke Exposure

Suryadinata, Rivan Virlando and Wirjatmadi, Bambang and Adriani, Merryana and Sumarmi, Sri (2021) The Expression Change of MMP-8 and Collagen Type-2 Intracell in Lung Tissue Due to Electronic Smoke Exposure. KEMAS: Jurnal Kesehatan Masyarakat, 17 (2). pp. 269-278. ISSN 1858-1196; E-ISSN 2355-3596

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Abstract

The number of electronic smokers has increased annually. Exposure to electronic cigarette will increase free radicals in the body and result in oxidative stress occurrence causing lung tissue damage. The severity degree of lung tissue damage caused by electronic smoke exposure depends on the duration of electronic cigarette smoke exposure and is going to affect Matrix Metalloproteinase-8 and collagen type-2 in the cells. The study aims to understand the change degree of Matrix Metalloproteinase-8 and collagen type- 2in lung tissue due to electronic cigarette smoke exposure. This study applied experimental method with post control group design. The male wistar rats were used as the animal models in this research in order to asses cell damage through the expression of Matrix Metalloproteinase-8 and collagen type-2 in the lung tissue using immunohisto- chemical staining. Exposure to electronic smoke cigarette was given to each group of animal models with difference in amount and time duration. The expression of Matrix Met- alloproteinase-8 indicated that there was a significant increase due to electronic smoke exposure (ANOVA, p=0.000). Meanwhile, the expression of collagen type-2 showed that there was a significant decrease because of electronic smoke exposure (ANOVA, p=0.000). Besides, MMP-8 and collagen type-2 manifested relationship existence and strong impact (r=0.948, p=0.000). The negative impact of exposure to electric cigarette smoke causes increased expression of Matrix Metalloproteinase-8 and decreased expression of type-2 collagen in lung tissue.

Item Type:	Article
Uncontrolled Keywords:	Matrik Metalloprotein 8, Collagen Tipe 2, E-Cigarette, Imunohistochemistry
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medical
Depositing User:	Ester Sri W. 196039
Date Deposited:	13 Dec 2021 06:41
Last Modified:	13 Dec 2021 07:47
URI:	http://repository.ubaya.ac.id/id/eprint/40816

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