TLC-based fingerprinting for Phyllanthus niruri from diverse geographical origins in East and Central Java Indonesia

Kartini, Kartini and Wulandari, Wilujeng Anissa and Jayani, Nikmatul Ikhrom Eka and Setiawan, Finna (2021) TLC-based fingerprinting for Phyllanthus niruri from diverse geographical origins in East and Central Java Indonesia. In: 4th International Conference on Biosciences (ICoBio 2021), 11th-12th August 2021, Bogor.

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Abstract

Phyllanthus niruri L. (meniran), the member of Euphorbiaceae, is a medicinal plant that is commonly found in tropical and sub-tropical areas such as Asia, America, and China. Various factors such as environment, geographical location, harvest time, and post-harvest process can affect the quality of crude drugs produced from P. niruri. The objective of this study was to evaluate the quality of meniran herbs obtained from 15 geographical origins in East and Central Java, Indonesia using Thin Layer Chromatography (TLC) profiles analyzed by chemometrics. TLC was carried out using TLC plate Si Gel 60 GF254 as stationary phase; toluene, ethyl acetate, methanol, 85% formic acid (75:25:25:6) as mobile phase; and visualized using NP/PEG Reagent. The results showed TLC-fingerprinting combined with chemometric (PCA and CA) analyses were able to discriminate the origin of P. niruri from different geographical origins. P. niruri from 15 locations of East and Central Java Indonesia were classified into 5 groups based on their chemical similarity. The samples that are grouped in one cluster have the similar quality of chemical compounds, while the samples in different clusters also have different qualities.

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	chemometric, geographical origin, herbal medicine, principal component analysis, TLC
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Kartini
Date Deposited:	17 Dec 2021 08:20
Last Modified:	03 Jun 2022 08:04
URI:	http://repository.ubaya.ac.id/id/eprint/40834

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