Rapid Screening Analysis of Antioxidant Activities in Green Tea Products Using DPPH and FRAP

Setiawan, Vendra and Phangestu, Sherlly and Soetikno, Agatha Grace and Arianti, Angelina and Kohar, Indrajati (2021) Rapid Screening Analysis of Antioxidant Activities in Green Tea Products Using DPPH and FRAP. Pharmaceutical Journal Of Indonesia, 7 (1). pp. 9-14. ISSN 2461-114X

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Official URL / DOI: https://pji.ub.ac.id/index.php/pji/article/view/74...


Introduction: Free radicals are formed inside and outside the body. Free radicals inside the zbody can be created in the cell compartments. The reactions are complex through the initiation, propagation, and termination stages. On the other hand, free radicals in the outer body can be formed from pollutions and environmental damage action. Excessive free radicals cause oxidative stress and use destructive cellular to affect health conditions. Antioxidants are known as essential agents in biological systems against diseases triggered by free radicals. One of the many antioxidants found in tea plants (Camellia sinensis) is polyphenols. Objectives: to describe the rapid screening analysis of the antioxidant activity methods using DPPH and FRAP in green tea drink. Methods: A colorimetry method was employed to evaluate the dominant antioxidant compound of the total phenolic and flavonoid content in two selected tea products. The total phenolic content was determined by utilizing the Folin-Ciocalteu reagent. While an aluminum colorimetric assay is applied to assess flavonoid content. Rapid screening of antioxidant activity using a spectrophotometric method is employed to determine IC50 values of tea extracts utilizing DPPH and FRAP. Results: Their IC50 score are 76,35 to 213,20 pm in loose leaves tea and 87,92 to 269,79 ppm in the teabag (DPPH method); and 9,88 until 26,95 ppm in loose leaves tea and 10,47 to 19,57 ppm in the teabag (FRAP method). Conclusions: The high total phenolic and flavonoid contents found in this experiment are in line with low IC50 values or in other words high antioxidant activities.

Item Type: Article
Uncontrolled Keywords: Antioxidant, Camellia sinensis, DPPH, FRAP
Subjects: R Medicine > RS Pharmacy and materia medica
R Medicine > RZ Other systems of medicine
S Agriculture > S Agriculture (General)
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: VENDRA SETIAWAN
Date Deposited: 03 Jan 2022 08:13
Last Modified: 27 Jan 2022 04:53
URI: http://repository.ubaya.ac.id/id/eprint/40877

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