Callus-mediated Somatic Embryogenesis and Plant Regeneration in Vanda tricolor Lindl.var.Pallida

Hardjo, Popy Hartatie and Savitri, Wina Dian and Artadana, Ida Bagus Made and Putra, Sulistyo Emantoko Dwi (2021) Callus-mediated Somatic Embryogenesis and Plant Regeneration in Vanda tricolor Lindl.var.Pallida. Jordan Journal of Biological Sciences, 14 (5). pp. 933-937. ISSN 1995-6673

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Abstract

In this study, a protocol to induce indirect somatic embryogenesis from the basal leaf segments of Vanda tricolor Lindl. var. Pallida has been developed. 2 0 T The experiments consisted of two stages، i.e. induction of SEs from calli and regeneration of plantlet from SEs. 2 0 T Embryogenic calli obtained from previous experiment (0.05 mg LP –1P NAA + 0.01 mg LP –1P BAP) were used to induce somatic embryos (SEs) on half-strength Murashige and Skoog (MS) medium supplemented with (0.05 mg L P –1 P toP P 0.20 mg LP –1P ) 6 -benzylaminopurine (BAP) alone or in combination with 0.01 mg L P –1 P α-naphtalene acetic acid P P (NAA). Embryogenic calli, those cultured on 0.05 mg LP –1P BAP and 0.01 mg LP –1 P NAA resulted in 90 % induction of SE structures at 30 d of culture period. Histological observation exhibited development of pro-embryo to form completed embryo. The pattern of SEs development started from embryogenic callus to form pro-embryo, followed by globular phase at 10 d of culture. Globular embryo elongated to form suspensor at 20 d of incubation period, and completed embryo. Regeneration of SEs into compl ete plantlets was attained on the half -strength MS medium without addition of any plant growth regulator (PGR). Based on the results of the present study, it can be concluded that 2 0 T half-strength MS medium supplemented with 2 0 T NAA 0.01 mg LP –1P R Rand BAP 0.05 mg LP –1P is the best medium for induction of SEs from embryogenic calli.

Item Type:	Article
Uncontrolled Keywords:	Embryogenic calli, Histology, Half-strength Murashige-Skoog medium, Micropropagation, Orchid, Pro-embryo
Subjects:	T Technology > T Technology (General)
Depositing User:	Ester Sri W. 196039
Date Deposited:	06 Jan 2022 07:18
Last Modified:	06 Jan 2022 07:27
URI:	http://repository.ubaya.ac.id/id/eprint/40920

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