The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies

Rissyelly, Rissyelly and Aziz, Syaikhul and Sangande, Frangky and Yodha, Agung Wibawa Mahatva and Budipramana, Krisyanti and Elfahmi, Elfahmi and Sukrasno, Sukrasno (2022) The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies. Hayati Journal of Biosciences, 29 (3). pp. 353-359. ISSN 1978-3019

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Abstract

Fern is one of the groups of primitive plants rich in secondary metabolites that are commonly used to treat various diseases, including antioxidant, antihyaluronidase, anti-inflammation, and respiratory disease but less investigated. Flavonoid is one of the secondary metabolites abundantly present in ferns. This study aims to isolate major compounds found in Blechnum orientale act as 15-lipoxygenase (15-LOX) inhibitors. Inhibition of lipoxygenase decrease the production of leukotriene that induces bronchoconstriction in asthma. Isoquercitrin (Quercetin-3-O-β-glucopyranoside) and trifolin (kaempferol-3-O-β-D-galactoside) have been successfully isolated from Blechnum orientale. Further in silico study was performed to explain the binding mode between flavonoid pyranoside or galactoside and flavonoid aglycone in the 15-LOX cavity and their amino acid residues interaction. Isoquercitrin binds with Ile663, Ile400, Leu408,Leu597, Ala404, and Arg403 in the 15-LOX cavity as a lipoxygenase inhibitor. Trifolin binds the same amino acids as isoquercetin with addition His366, Gln596,and Phe175. Both isoquercitrin and trifolin act as competitive inhibitors against lipoxygenase enzymes.

Item Type: Article
Uncontrolled Keywords: Fern, Flavonoid, In silico, Isoquercitrin, Kaempferol, Lipoxygenase
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: KRISYANTI BUDIPRAMANA
Date Deposited: 08 May 2022 13:52
Last Modified: 08 May 2022 13:52
URI: http://repository.ubaya.ac.id/id/eprint/41814

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