Immobilization of xylanase on acid pretreatment bentonite as green biocatalyst

Riadi, Lieke and Chrisnasari, Ruth and Kristanto, Joshua and Bigravida, Cahaya Caesar and Sanoe, Meyta (2022) Immobilization of xylanase on acid pretreatment bentonite as green biocatalyst. In: AIP Conference Proceedings 2470, 3rd BIANNUAL INTERNATIONAL CONFERENCE ON INFORMATICS, TECHNOLOGY, AND ENGINEERING 2021 (InCITE 2021): Leveraging Smart Engineering, 25–26 August 2021, Surabaya, Indonesia (Online).

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Hydrochloric acid was used to prepare bentonite carrier for xylanase immobilization from Trichoderma reesei as green biocatalyst. The process to produce xylanase from Reutealis trisperma was carried out for 72 hours in batch fermentation at initial pH of 6.0. The xylanase activity was 205.9±0.84 U/mL. It was then purified and analyzed using SDS-PAGE and found to be 26 and 32 kDa. The purification yield was 31.32 %. The bentonite carrier with 140 mesh particle size was pretreated at various HCl concentration. The impurities consist of several ions were successfully replaced by H+ ion as confirmed by FTIR spectra. The carrier was used to immobilize xylanase and the best carrier used in immobilization was bentonite with 2M HCl concentration pretreatment. The best final immobilization was 87.765 % and the leaching was 0.059 %. The biocatalyst can be proposed as green biocatalyst to reduce chlorine used in pulp bleaching.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: xylanase, immobilization, bentonite, green catalyst
Subjects: T Technology > TP Chemical technology
Divisions: Faculty of Engineering > Department of Chemical Engineering
Depositing User: Lieke Riadi 6116
Date Deposited: 30 May 2022 04:57
Last Modified: 28 Mar 2023 03:43

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