Comparative Study Of In-Vitro Toxicity Of Pure Honey And Fermented Honey Using The Bslt (Brine Shrimp Lethality Test) Method

Wulansari, Devyani Diah and Wulandari, Devyana Dyah and Krisdayanti, Affina (2022) Comparative Study Of In-Vitro Toxicity Of Pure Honey And Fermented Honey Using The Bslt (Brine Shrimp Lethality Test) Method. Medical Technology and Public Health Journal, 6 (2). pp. 148-156. ISSN 2549-189X; e-ISSN 2549-2993

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Abstract

Honey is an alternative product used in traditional medicine because of its many benefits, honey is a sweet liquid made by bees using flower nectar. The purposed of this study was to determine the phytochemical screening and the potential for acute toxicity of pure honey and fermented honey to Artemia salina larvae using the BSLT method (Brine Shrimp Lethality Test) as indicated by the LC50 value. This experimental study used 540 Artemia salina larvae which were divided into 1 negative control, 1 positive control and 7 groups of pure honey and fermented honey concentration series, each consisting of 10 larvae with replication 3 times for each treatment. The concentrations of pure honey and fermented honey were 1000, 750, 500, 100, 50, 10, 1 ppm, respectively. The LC50 value was determined using a probit analysis after the percentage of fatalities findings were obtained. Pure honey yielded a toxicity of 59.75 μg/mL. While the fermented honey obtained an LC50 value of 3.28 μg/mL, which means the LC50 value was 30 ppm, indicating that the fermented honey sample was included in the very toxic category. Toxicity test on pure honey showed the LC50 value was in the toxic range. In the toxicity test, the fermented honey showed an LC50 value of 30 ppm, which means that the fermented honey has a very toxic toxicity value. There are differences in LC50 values in the second sample. However, statistically, there is no significant difference between pure honey and fermented honey

Item Type:	Article
Uncontrolled Keywords:	BSLT,fermented honey,pure honey,toxicity
Subjects:	Q Science > Q Science (General) R Medicine > RB Pathology R Medicine > RZ Other systems of medicine
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	DEVYANI DIAH WULANSARI
Date Deposited:	21 Dec 2022 04:26
Last Modified:	06 Nov 2023 04:30
URI:	http://repository.ubaya.ac.id/id/eprint/43008

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