Effect of organic additives on regeneration of orchid hybrid (Dendrobium ‘Bertachong’ X Dendrobium ‘Blackspider X Sutiknoi’)

Wijaya, Agnes Natalia and Artadana, Ida Bagus Made and Putra, Sulistyo Emantoko Dwi and Hardjo, Popy Hartatie (2023) Effect of organic additives on regeneration of orchid hybrid (Dendrobium ‘Bertachong’ X Dendrobium ‘Blackspider X Sutiknoi’). In: AIP Conference Proceedings 2606 Proceedings of the 8th International Symposium of Innovative Bioproduction Indonesia on Biotechnology and Bioengineering 2021, 15-16 November 2021, Bogor.

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Abstract

Dendrobium is a genus of orchids that has been widely planted, both domestically and internationally. Orchid conventional breeding is generally done to produce new variants, but seeds from crosses are generally difficult to germinate. To solve this problem, tissue culture has long been used to germinate orchid seeds. This study aims to obtain a suitable medium composition for growing seeds from crosses of Dendrobium 'Bertachong' X Dendrobium 'Blackspider x Sutiknoi'. The seeds were grown on two different media types, MS (Murashige-Skoog) and VW (Vacin-Went), with the addition of 2% sucrose and with or without the addition of organic extracts (either mungbean sprouts extract or coconut water). Plantlets were sub-cultured every 4 weeks for 2 times on the same medium but with the addition of activated charcoal, and followed by a third subculture with the addition of 1 mg.L-1 NAA (Naphthalene Acetic Acid) in each medium. The best growth shown by fast regeneration and good seedling quality (multiple buds and roots formed) was obtained on MS medium with the addition of 2% sucrose and 15% mungbean sprouts extract.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	T Technology > T Technology (General)
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Ester Sri W. 196039
Date Deposited:	12 Jan 2023 06:46
Last Modified:	25 Jul 2024 02:50
URI:	http://repository.ubaya.ac.id/id/eprint/43107

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