Anti-inflammatory activity of Lactobacillus spp. and Rhodopseudomonas palustris probiotics

Kok, Tjie (2023) Anti-inflammatory activity of Lactobacillus spp. and Rhodopseudomonas palustris probiotics. Bioactive Compounds in Health and Disease (BCHD), 6 (4). pp. 63-72. ISSN 2574-0334(Online); 2769-2426(Print)

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Abstract

Background: Probiotics have been used for many years to promote human health by mitigating inflammation. However, their mechanisms have not been fully elucidated. During inflammation, excessive and/or prolonged production of pro- inflammatory cytokines is related with various inflammatory diseases and cancer. Several probiotics have been reported as playing a role in suppressing the level of pro-inflammatory cytokines, as the human body attempts to recover itself. The aim of this study was to evaluate the anti-inflammatory activity of probiotics consisting of Lactobacillus spp. and Rhodopseudomonas palustris on macrophage RAW 264.7 cells. Methods: The probiotics mixture was centrifuged to separate supernatant, i.e., the probiotics extract, from the cells. The probiotics extract was then evaluated for its effects on cell viability and for anti-inflammatory activity of LPS inflammation-induced RAW 264.7 cells. Results: The results showed that the probiotics extract was able to decrease the level of IFN-γ, IL-1β, TNF-α, IL-8, TGF-β1 pro-inflammatory cytokines or mRNAs, and increase the level of IL-10 anti-inflammatory mRNA. Conclusion: The probiotics extract was identified to have anti-inflammatory activity. Thus, the probiotics are promising to be used for treatment of chronic inflammatory conditions.

Item Type:	Article
Uncontrolled Keywords:	probiotics, Lactobacillus spp., Rhodopseudomonas palustris, RAW 264.7 cells, anti-inflammatory activity
Subjects:	T Technology > T Technology (General)
Divisions:	Postgraduate Programs > Master Program in Biotechnology
Depositing User:	Ester Sri W. 196039
Date Deposited:	26 May 2023 04:15
Last Modified:	07 Jul 2023 02:57
URI:	http://repository.ubaya.ac.id/id/eprint/44210

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