Comparison of mobility shift affinity capillary electrophoresis and capillary electrophoresis frontal analysis for binding constant determination between human serum albumin and small drugs

Mlcochova, Hana and Ratih, Ratih and Michalcova, Lenka and Watzig, Hermann and Glatz, Zdenek and Stein, Matthias (2022) Comparison of mobility shift affinity capillary electrophoresis and capillary electrophoresis frontal analysis for binding constant determination between human serum albumin and small drugs. Electrophoresis, 43 (16-17). pp. 1724-1734. ISSN 1522-2683

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Abstract

In this study, two capillary electrophoresis–based ligand binding assays, namely, mobility shift affinity capillary electrophoresis (ms-ACE) and capil- lary electrophoresis-frontal analysis (CE-FA), were applied to determine binding parameters of human serum albumin toward small drugs under similar exper- imental conditions. The substances S-amlodipine (S-AML), lidocaine (LDC), l-tryptophan (l-TRP), carbamazepine (CBZ), ibuprofen (IBU), and R-verapamil (R-VPM) were used as the main binding partners. The scope of this comparative study was to estimate and compare both the assays in terms of their primary mea- sure’s precision and the reproducibility of the derived binding parameters. The effective mobility could be measured with pooled CV values between 0.55% and 7.6%. The precision of the r values was found in the range between 1.5% and 10%. Both assays were not universally applicable. The CE-FA assay could successfully be applied to measure the drugs IBU, CBZ, and LDC, and the interaction toward CBZ, S-AML, l-TRP, and R-VPM could be determined using ms-ACE. The aver- age variabilities of the estimated binding constants were 64% and 67% for CE-FA and ms-ACE, respectively.

Item Type:	Article
Uncontrolled Keywords:	binding parameters, capillary electrophoresis-frontal analysis, drugs, human serum albumin, mobility shift affinity capillary electrophoresis
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	05 Jun 2023 04:19
Last Modified:	27 Sep 2023 07:36
URI:	http://repository.ubaya.ac.id/id/eprint/44231

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