Dissolved oxygen on xylanase production by Trichoderma reesei using Reutalis trisperma press cake as an additional substrate

Riadi, Lieke and Agustin, Yuana Elly and Chrisnasari, Ruth and Kok, Tjie and Sanoe, Meyta (2023) Dissolved oxygen on xylanase production by Trichoderma reesei using Reutalis trisperma press cake as an additional substrate. Asia Pacific Journal of Molecular Biology and Biotechnology (APJMBB), 31 (2). pp. 109-116. ISSN 0128-7451, eISSN:2672-7277

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Abstract

Dissolved oxygen (DO) level in media plays a big role in the succession of the fermentation process by a microorganism. In this project, Trichoderma reesei was cultivated in two types of media, media A and B, both in 250 mL shake flask and 1.5 L fermenter at varying DO level. Media A and B contained the same carbon source and mineral salts, the difference between both media was the presence of press cake in Media B as urea and ammonium sulfate replacement, whereas the nitrogen sources in media A consists of urea and ammonium sulfate. The batch fermentation process was conducted in a 7.8 C/N ratio with an initial pH of 6. The purified enzyme was shown to convert xylan to xylose. Media B that contained Reutealis trisperma press cake gives a higher xylanase activity than media A without press cake, for both shake flask and fermenter cultivations. A xylanase activity of 101.07±1.65 U/mL and 39.30±0.64 U/mL was produced in a shake flask under 140 rpm for 96 hours of fermentation with ratio of 0.6 media B/flask volume and 0.6 media A/flask volume, respectively. A xylanase activity of 205.90±0.84 U/mL and 88.87±1.45 U/mL was produced in 72 hours at 0.8 L/min of aeration rate in a 1.5 L fermenter with media A and media B, respectively. Thus, this study identified the Reutealis trisperma cake can be used as a beneficial additional substrate and nitrogen sources. The increase in DO level, which indicated by increasing aeration rate, can generate a higher xylanase activity for both media in shake flask and fermenter.

Item Type:	Article
Uncontrolled Keywords:	dissolved oxygen, press cake, purification, Reutealis trisperma, xylanase
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Engineering > Department of Chemical Engineering
Depositing User:	Ester Sri W. 196039
Date Deposited:	06 Jul 2023 01:47
Last Modified:	06 Jul 2023 02:15
URI:	http://repository.ubaya.ac.id/id/eprint/44439

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