Isolation based on antioxidant activity of 80% ethanolic extract faloak (Sterculia quadrifida R. Br.) stem bark

Ruskim, Jennifer and Budiono, Ryanto and Kartini, Kartini and Oktaviyanti, Nina Dewi and Setiawan, Finna (2023) Isolation based on antioxidant activity of 80% ethanolic extract faloak (Sterculia quadrifida R. Br.) stem bark. Jurnal Ilmiah Farmasi (JIF). pp. 102-115. ISSN 1693-8666, E-ISSN 2657-1420

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Abstract

Background: Free radicals consist of one or more unpaired electrons in an outer orbit that causes them to be more reactive. This will lead to oxidative stress, which is one of the major causes of degenerative diseases such as cancer, diabetes mellitus, and stroke. Therefore, consuming sufficient antioxidants can prevent oxidative stress. Faloak (Sterculia quadrifida R. Br.) is one of the endemic medicinal plants in East Nusa Tenggara and is a resource of antioxidants that are supported by scientific evidence. Until now, the information about the antioxidant compounds in Faloak was limited. Objective: This study aims to identify active compounds in an 80% ethanol extract from Faloak bark as antioxidants based on their TLC-bioautography profiles using an ABTS assay. Method: The methods of this study start with the preparation of simplicia, fractination using kinetic maceration, compound group identification and TLC-bioautography, separation using column chromatography [stationary phase: silica H60; mobile phase: 2-propanol: chloroform: acetic acid glacial (5:1:0,5)], identification using a UV-Vis spectrophotometer, and antioxidant activity determination of the extract and isolate using an ABTS assay. Result: Through this study, a subfraction (SFFE A) with a brown-red colour and a blue spot under UV 365 nm and an Rf value of 0.65 was obtained. This subfraction (SFFE A) was predicted to be a polyphenol compound. The inhibition percentages of 125, 250, and 500 ppm successively are 80,93%; 95,54%; and 96,69%. Conclusion: The active isolate of ethanol extracting 80% of the faloak stem bark is suspected to be a polyphenol group and has strong antioxidant activity.

Item Type:	Article
Uncontrolled Keywords:	faloak (Sterculia quadrifida R. Br.), antioxidants, isolation, kinetic maceration, column chromatography
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	11 Sep 2023 03:52
Last Modified:	11 Sep 2023 03:52
URI:	http://repository.ubaya.ac.id/id/eprint/44901

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