Synthesis and in vitro activity tests of N-benzoyl-N'-phenylthiourea derivatives as macrophage migration inhibitory factor [Síntesis y pruebas de actividad in vitro de derivados de N-benzoil-N'-feniltiourea como factor inhibidor de la migración de macrófagos]

Kesuma, Dini and Putra, Galih Satrio and Yuniarta, Tegar Achsendo and Suhud, Farida and Sumartha, I Gede Ari and Boengas, Sawitri and Sulistyowaty, Melanny Ika and Kok, Tjie (2023) Synthesis and in vitro activity tests of N-benzoyl-N'-phenylthiourea derivatives as macrophage migration inhibitory factor [Síntesis y pruebas de actividad in vitro de derivados de N-benzoil-N'-feniltiourea como factor inhibidor de la migración de macrófagos]. Journal of Pharmacy & Pharmacognosy Research (JPPRes), 11 (5). pp. 902-925. ISSN 0719-4250

PDF Dini Kesuma_Synthesis and in vitro activity tests.pdf Download (6MB)

Abstract

Context: The COVID-19 pandemic in 2020 resulted in widespread mortalities due to cytokine storms in the affected patients. Macrophage migration inhibitory factor (MIF) is one of the most interesting targets in developing anti-COVID-19 drugs. Some thiourea compounds have been identified as having potential as MIF inhibitors. Aims: To investigate MIF inhibitory activity of N-benzoyl-N'-phenylthiourea derivatives. Methods: The study consists of in-silico activity prediction of designed compounds using a molecular docking approach against MIF protein (PDB ID: 1LJT). Afterwards, the designed compounds were synthesized and tested in vitro using the tautomerase activity approach. Results: The molecular docking study showed that all designed compounds possess comparable docking scores to the native ligand of the protein. MIF Assay performed on compounds (1) and (2) indicated a decrease in tautomerase activity of the MIF target protein of only 10.1 and 6.2%, respectively, compared to the positive control. Conclusions: In silico results predicted better bioactivity against MIF protein, but the result does not translate to the in vitro assay, where two of the designed compounds possess only low inhibitory activity.

Item Type:	Article
Uncontrolled Keywords:	1LJT; MIF assay; tautomerase activity; thiourea derivatives
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	13 Sep 2023 03:32
Last Modified:	13 Sep 2023 03:32
URI:	http://repository.ubaya.ac.id/id/eprint/44916

Actions (login required)

View Item