Hydrogen Peroxide Plasma Sterilization Sabotages the Efficacy of Lidocaine HCl Injection

Aprilia, Dini and Sumiyani, Ririn and Tripuspitasari, Niniek and Pambudi, Ade Muchlas Wijayanto Dwi and Avanti, Christina (2023) Hydrogen Peroxide Plasma Sterilization Sabotages the Efficacy of Lidocaine HCl Injection. The Open Anesthesia Journal, 17.

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Abstract

Background: Lidocaine injection with 2% HCl as an anesthetic drug must guarantee its sterility to avoid microbial contamination. In efforts to maintain the sterile preparation of Lidocaine HCl 2% before use in hospitals, some anesthesiologists opt for re-sterilization. Objective: This study aimed to evaluate the impact of plasma sterilization using hydrogen peroxide on Lidocaine HCl levels employing a validated Ultra Performance Liquid Chromatography (UPLC) assay. Methods: The 2% Lidocaine HCl samples were separated into two groups, one undergoing re-sterilization with hydrogen peroxide and the other handled only with aseptic techniques. The chromatographic assay was performed using a Waters Corp Acquity UPLC® H-Class system and a Waters Corp Acquity UPLC® BEH C18 column, with a mobile phase of 20% Acetonitrile and 80% Acetate Buffer pH 3.4, flow rate of 0.3 mL/min, and total duration of 4.5 minutes. Results: The results showed a decrease in Lidocaine HCl levels to 1.88% after re-sterilization and 2.01% without re-sterilization. Conclusion: These findings suggest that re-sterilization with hydrogen peroxide plasma sterilization leads to a significant decrease in Lidocaine HCl levels, causing non-compliance with pharmacopoeia standards.

Item Type:	Article
Uncontrolled Keywords:	Hydrogen peroxide, Plasma sterilization, Lidocaine HCl, Anesthetic drugs, Sterility, UPLC
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	16 Oct 2023 08:18
Last Modified:	08 Jan 2024 01:56
URI:	http://repository.ubaya.ac.id/id/eprint/45121

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