Decreasing α-synuclein Aggregation by Ethanol Extract of Keluwih (Artocarpus camansi) Leaves on Rotenone- Induced Adult Zebrafish as Parkinson’s Diseases Model

Gondokesumo, Marisca Evalina and Muslikh, Faisal Akhmal and Nopitasari, Ni Putu Diah and Putri, Putu Dea Angelita (2023) Decreasing α-synuclein Aggregation by Ethanol Extract of Keluwih (Artocarpus camansi) Leaves on Rotenone- Induced Adult Zebrafish as Parkinson’s Diseases Model. Jurnal Teknologi Laboratorium, 12 (2). pp. 53-60. ISSN 2338-5634; E-ISSN 2580-0191

PDF Marisca Evalina Gondokesumo_Decreasing α-synuclein Aggregation.pdf Download (10MB)

Abstract

The prevalence of Parkinson's disease is increasing every year. This progressive disease is characterized by the loss of neurons in the substantia nigra due to the presence of α- synuclein aggregates. Keluwih leaves (Artocarpus camansi) are known to have activity in inhibiting acetylcholinesterase, as well as being an antioxidant and anti-inflammatory. The aim of this study was to evaluate the effect of ethanol extract of A. camansi leaves on the levels of α-synuclein in male and female adult zebrafish induced with rotenone. The zebrafish were induced with rotenone at a concentration of 5 μg/L for 28 days, along with the administration of 96% ethanol extract of A. camansi leaves at doses of 2.5, 5, 7.5, or 10 mg/L. The media was changed every 48 hours to maintain the concentration of rotenone and extract. After 28 days, α-synuclein levels were examined using immunohistochemistry. The administration of ethanol extract of A. camansi leaves can reduce the average levels of α-synuclein in male and female adult zebrafish, with the optimum dose being 2.5 mg/L. Therefore, it can be concluded that the administration of ethanol extract of A. camansi leaves can be used as an alternative treatment for Parkinson's disease.

Item Type:	Article
Uncontrolled Keywords:	α-synuclein, Artocarpus camansi, Parkinson disease, Zebrafis
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	02 Jan 2024 07:52
Last Modified:	02 Jan 2024 07:52
URI:	http://repository.ubaya.ac.id/id/eprint/45571

Actions (login required)

View Item