Explant surface sterilization protocol for micropropagation of Amorphophallus muelleri Blume

Irawati, Fenny and Wijaya, Agnes Natalia and Manurung, Anggi and Thongiratama, Michael Anthony and Savitri, Wina Dian and Hardjo, Popy Hartatie (2023) Explant surface sterilization protocol for micropropagation of Amorphophallus muelleri Blume. In: Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022), 24-25 August 2022, Surabaya, Indonesia (Online).

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Official URL / DOI: https://www.atlantis-press.com/proceedings/nrls-bi...


The success of tissue culture is greatly influenced by the explant surface sterilization technique. The presence of bacterial and fungi contamination, and the occurrence of browning on the explants can interfere with the process of culture propagation. High concentration of sterilant agents will inhibit cell division and the growth of explant tissues. This study aims to determine the optimum type and concentration of sterilant agents and how to use them to sterilize explants without causing much damage to explant tissue. The surface sterilization treatment of bulbil explant by soaking in a 250 mg.L-1 cefotaxime antibiotic solution for 30 min, and followed by immersion in 0.2% HgCl2 for 7 min, followed by soaking in NaOCl 1:2 for 10 min showed the best results obtained 60% sterile explants and faster growth of callus cells from explants. Callus grows to form adventitious buds within a period of 7 weeks.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: aseptic technique; bulbil; sterilant agent; sterile explant
Subjects: T Technology > T Technology (General)
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Ester Sri W. 196039
Date Deposited: 03 Jan 2024 04:53
Last Modified: 04 Jan 2024 02:49
URI: http://repository.ubaya.ac.id/id/eprint/45589

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