Changes of histopathology and PPAR-ɣ gene expression in hyperglycaemia-mice

Sukweenadhi, Johan and Ikawaty, Risma and Anne Marie, Yohanes Bosko and Humardani, Farizky Martriano and Mulyanata, Lisa Thalia and Adeodata, Lady Theresa and Emantoko, Sulistyo (2024) Changes of histopathology and PPAR-ɣ gene expression in hyperglycaemia-mice. In: 7th International Conference Medical and Health Science 2023(ICMHS 2023), August 8th-9th 2023, Universitas Muhammadiyah Yogyakarta, Bantul, Yogyakarta.

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Abstract

Type 2 diabetes mellitus is multifactorial disease characterized by hyperglycaemia condition that can be induced by high diets in carbohydrates and/or MSG. Diabetic nephropathy is a complication that often occurs in DM patients. Environmental and genetic factors play roles in histopathological changes in kidney organs and changes in gene expression responsible for glucose metabolism. The aim of the study is to examine the effect of high diet carbohydrate and/or MSG on changes in kidney histopathology and PPAR-ɣ gene expression. Forty Swiss Webster male mice with the age of 7-weekold were divided into 1 control group (A) and 3 groups induced with high carbohydrate 71% (B), MSG 10% (C), mixture of carbohydrates 71% and MSG 10% (J) for 14 weeks. Termination was conducted to remove the kidneys for histopathological procedures and muscle to shows changes in PPAR-ɣ gene expression through polymerase-chain reaction (PCR). Both results analysed with ImageJ application and tested with one-way anova. The results is thickening of glomerular basement membrane (GBM) was found which characterized by narrowing capsular spaces in group B (0,013μm), group C(0,010μm), and group J(0,008μm) compared to group A (0,016μm) with significant results between group p value < 0,05. Average ratio PPAR-y gene expression values to housekeeping gene GAPDH in group A (1,48), group B (1,38), group C (1,30) and group J (1,37) with non-significant difference between groups p value > 0.05. Hyperglycaemia condition induced by high diet carbohydrates and/or MSG resulted in thickening GBM in kidneys, whereas expression of PPAR-ɣ gene in the muscle did not show significant change.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	Q Science > QL Zoology R Medicine > RB Pathology R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	JOHAN SUKWEENADHI
Date Deposited:	24 Jun 2024 04:21
Last Modified:	24 Jun 2024 04:21
URI:	http://repository.ubaya.ac.id/id/eprint/46630

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