Gene Expression Data Analysis In Response To Nicotine Exposure: A Literature Study Revealing Differentially Expressed Genes In Biological Pathways

Munawwarah, Munawwarah and Azminah, Azminah (2023) Gene Expression Data Analysis In Response To Nicotine Exposure: A Literature Study Revealing Differentially Expressed Genes In Biological Pathways. The Journal of World Science, 2 (12). pp. 2015-2031. ISSN 2828-8726; E-ISSN 2828-9307

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Abstract

Nicotine is the main active chemical component in cigarette smoke that causes addiction. Prolonged exposure to nicotine has been confirmed to cause changes in gene expression related to metabolic functions and neural modifications in the brain. This study aims to analyze receptor targets resulting from nicotine exposure through Differentially Expressed Genes (DEG) analysis and to identify secondary metabolites in basil essential oil (Ocimum basilicum L.) using GC-MS, as well as to predict potential activity against specific receptors and target therapeutic compounds in silico (molecular docking and molecular dynamics). DEG analysis identified the nicotinic acetylcholine receptor (nAChRs) subtype α4-β2 with PDB code 6PV7 as the receptor target. GC-MS compound identification revealed 31 secondary metabolites in basil essential oil. ADMET and molecular docking analyses identified beta-bisabolene as the target therapeutic compound. The predicted potential activity of beta-bisabolene against the nicotinic acetylcholine receptor (nAChRs) subtype α4-β2 showed a compact structure and better stability than the drug. These findings support the potential of basil essential oil as an alternative therapy to mitigate the negative effects of nicotine exposure. Keywords: Differentially Expressed Genes, In silico, nAChRs, beta-bisabolene

Item Type:	Article
Uncontrolled Keywords:	nicotine, gene expression, differentially expressed genes
Subjects:	R Medicine > RM Therapeutics. Pharmacology T Technology > TP Chemical technology
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Munawwarah
Date Deposited:	23 Aug 2024 08:14
Last Modified:	23 Aug 2024 08:14
URI:	http://repository.ubaya.ac.id/id/eprint/46987

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